《植物生理学报》 2013, 49(2): 167-174

五种菌草苗期对碱胁迫的生理响应及抗碱性评价

林兴生^1,3, 林占熺^1,3,*, 林辉^1,3, 林冬梅^1,3, 罗海凌^1,3, 胡应平^1,3, 林春梅^1,3, 朱朝枝^2,*

福建农林大学1菌草研究所, 2金山学院, 福州350002; 3国家菌草工程技术研究中心, 福州350002

通信作者：林占熺；E-mail: wittylin@163.com；Tel: 0591-83768494

摘　要：

为探明莱竹、芦竹、巨菌草、象草、稗草5种菌草对碱胁迫的生理响应及抗碱能力, 分别以0、50、100、125、150、200 mmol·L-1 Na2CO3溶液模拟碱胁迫, 测定叶片细胞膜透性, 丙二醛(MDA)和可溶性糖(SS)含量, 超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性, 以及恢复试验后植株死亡率, 并利用隶属函数法对以上指标进行综合评价。结果表明, 随Na2CO3胁迫浓度的升高, 5种菌草的相对电导率、MDA含量呈上升趋势, SS含量, SOD、POD、CAT活性呈先升高后下降的趋势; 稗草、莱竹、象草、巨菌草、芦竹的生理指标平均隶属函数值分别为0.36、0.95、0.01、0.14、0.83, 半致死Na2CO3胁迫浓度分别为107.0、127.7、68.5、78.5、123.0 mmol·L-1; 5种菌草的抗碱能力强弱为: 莱竹>芦竹>稗草>巨菌草>象草。

关键词：菌草; 生理响应; 碱胁迫; 抗碱性

收稿：2012-11-22   修定：2013-01-17

资助：国家林业公益性行业科研专项(201004019)、科技部国家中心组建项目(2011FU125X11)和福建省科技重大专项(2012NZ0002)。

Physiological Responses and Alkaline-Tolerance Evaluation on 5 Species of Juncao under Alkaline Stress during Seedling Stage

LIN Xing-Sheng^1,3, LIN Zhan-Xi^1,3,*, LIN Hui^1,3, LIN Dong-Mei^1,3, LUO Hai-Ling^1,3, HU Ying-Ping^1,3, LIN Chun-Mei^1,3, ZHU Chao-Zhi^2,*

¹Juncao Research Institute, ²Jinshan College, Fujian Agriculture and Forestry University, Fuzhou 350002, China; ³National Engi-neering Research Center of Juncao, Fuzhou 350002, China

Corresponding author: LIN Zhan-Xi; E-mail: wittylin@163.com; Tel: 0591-83768494

Abstract:

In order to verify the physiological responses and alkaline tolerance of 5 species of juncao, Echi-nochloa crusgalli, Arundo sp., Pennisetum purpureum, Pennisetum sp. and Arundo donax, the physiological in-dexes of juncao leaves under alkaline stress (Na2CO3) with different concentrations of 0, 50, 100, 125, 150 and 200 mmol·L-1 for 5 d, including contents of malondialdehyde (MDA) and soluble sugar (SS), activities of su-peroxide dismutase (SOD), peroxidase (POD) and catalase (CAT), and membrane permeability, were deter-mined respectively. After recovery test, the mortality rates of the 5 juncao species were determined. The all in-dexes were evaluated by using subordinate functions. The result indicated that when the concentration of Na2CO3 was increased, SS content and activities of SOD, POD and CAT of the 5 species of juncao were first in-creased and then decreased, and the relative electrical conductivity (REC) and MDA content of the 5 juncao species were increased. The half-lethal concentrations of Na2CO3 solution of E. crusgalli, Arundo sp., P. pur-pureum, Pennisetum sp. and A. donax were 107.0, 127.7, 68.5, 78.5 and 123.0 mmol·L-1, respectively. Their av-erage membership function values were 0.36, 0.95, 0.01, 0.14 and 0.83, respectively. The alkaline tolerance of 5 juncao species were Arundo sp.>A. donax>E. crusgalli>Pennisetum sp.>P. purpureum.

Key words: juncao; physiological response; alkaline stress; alkaline tolerance